http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2870557-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49f9482d0ae56f3c77198101cae760a9 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-162 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-301 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-1015 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-179 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-201 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1055 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
| filingDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1220d216e3e241900f7d978a5a2d0c84 |
| publicationDate | 2021-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2870557-T3 |
| titleOfInvention | Method to determine the levels of interactions between biomolecules |
| abstract | Method for determining the levels of interaction between biomolecules, such as proteins, in a sample, comprising providing a first and a second information-bearing oligonucleotide (PI), wherein the first and second PI oligonucleotides are bound, covalently or non-covalently , to a first and a second affinity reagent, such as antibodies, that have the ability to bind to a first and a second biomolecule, in which the first and second PI oligonucleotides each comprise at least one chain stretch single that is complementary to a portion of another information receptor (IR) oligonucleotide or an activator oligonucleotide; in this way, after the hybridization of at least one section of the single chain in at least one of the first and second oligonucleotides PI to its complementary part of another oligonucleotide, it is possible to measure the relative proportion of the first and second biomolecules interacting and non-interacting in the sample at the single cell or single molecule level, wherein the single-stranded stretch of the first and second PI oligonucleotides allows interaction, (a) between the first and/or second PI oligonucleotides and a information receptor (IR) oligonucleotide, wherein the RI oligonucleotide carries at least two cleavage motif sites which, upon hybridization with a PI oligonucleotide, become double-stranded to allow cleavage, or (b) between the first and/or second PI oligonucleotide and a trigger oligonucleotide, wherein the PI oligonucleotides connected to said antibodies are designed to contain hairpin structures, and wherein said PI oligonucleotides are modified with fluorophores and inhibitors, which are positioned such that only one fluorophore per oligonucleotide can emit light, wherein hybridization between the first and/or second PI oligonucleotide and the activator oligonucleotide rearranges the positions of the fluorophores and inhibitors, such that the signaling fluorophore allows discrimination between interacting and non-interacting biomolecules. |
| priorityDate | 2017-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 141.