http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2869895-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59e1d955a7b1560dc68cf7fd800e8fac |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 |
| filingDate | 2002-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b56f01ed50d4a1662866cf4e96445fe0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b974c4c2c138583eeef1ab671fe4f79 |
| publicationDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2869895-T3 |
| titleOfInvention | Protein purification method |
| abstract | A method for removing contaminating DNA in a sample containing antibodies comprising the following steps: 1) applying the sample containing the antibody to Protein A affinity chromatography, followed by an elution step involving i) equilibration of the Protein A resin using 1 M NaCl / 10 to 100 mM citrate-phosphate buffer at pH 7.5; ii) loading the antibody sample onto the resin, followed by iii) a first washing step using 1 M NaCl / 10 to 100 mM citrate-phosphate buffer pH 7.5, and iv) a second washing step using 10 to 100 mM citrate-phosphate buffer pH 7.5, followed by v) an elution step using 2.5 mM HCl pH 2.6; 2) adjust the pH of the eluate to pH 7 with a 300 mM Tris solution to form particles; and 3) removing the resulting particles, wherein the antibody is the humanized anti-IL-6 receptor hPM-1 antibody. |
| priorityDate | 2001-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 292.