http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2848032-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0471e47f3a2760e9befddc375477afa7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5023 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5047 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2015-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b13a9ec20c537b34bce7b44ad4d1ac9d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90d400e3cdbc5685ce2ce028ea8514cf |
| publicationDate | 2021-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2848032-T3 |
| titleOfInvention | Method of retrieving two or more genes, or gene products, that code for an immunoreceptor |
| abstract | Method comprising the following sub-stages: recovering two or more genes, or gene products, or cDNAs, that code for an immunoreceptor that has two or more subunits, two or more genes, or gene products, that are comprised in a given source cell, method comprising the following steps: a) encapsulating source cells together with an mRNA capture moiety in microreactors, in such a way that each microreactor comprises a source cell and in which the mRNA capture moiety is a bead or b-particle ) lyse the cells in the microreactors, release the cellular mRNA into the lumen of the microreactors and subsequently bind it to the mRNA capture moiety c) lyse or disrupt the microreactors and reverse transcribe the mRNA bound to the capture residues of MRNA in solution to obtain the corresponding cDNA gene products d) create a construct that encompasses the two or more gene products that encode the immunoreceptor subunits tor, in which creating a construct that encompasses the two or more gene products that encode the immunoreceptor subunits is achieved by amplifying the cDNA by overlapping extension PCR within microreactors, in which each microreactor contains a single capture moiety of mRNA resulting from step c), and e) cloning the PCR product in a plasmid vector to obtain a bi- or multicistronic expression construct; f) thus creating a library of plasmids containing the cDNA encoding the immunoreceptors of a given source cell population, and creating a library of expressing cells, a library in which each cell can express two or more genes, or gene products, encoding the recovered immunoreceptor subunits, in which creating the library comprises the following steps: g) transfecting expressing cells with a bi or multicistronic expression construct, and h) encapsulating expressing cells in microreactors with one expressing cell per microreactor, and i) culturing the encapsulated cells under conditions that allow the expression of proteins and / or proliferation of the cells. |
| priorityDate | 2014-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 171.