http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2843558-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_459549086df37b5bed0003502de7e7aa |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-522 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-22 |
| filingDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34c3d384a859eae51a8608595e027359 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2e43fdbb85d2fb105d9db1c537f8bbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f65554ceb62adf78377add623c007e5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c2d0d221c70522e948ac5f74dd93fe4 |
| publicationDate | 2021-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2843558-T3 |
| titleOfInvention | Improved refolding process of antibody fragments |
| abstract | A process for recovering refolded ranibizumab, the process comprising the steps of: a) isolating inclusion bodies comprising a ranibizumab light chain and heavy chain from the bacterial host cell; b) solubilizing said inclusion bodies comprising a ranibizumab light chain and / or heavy chain in a first buffered solution comprising 10 mM Tris-Cl to 60 mM, 6 M to 8 M urea and DTT at a pH in the range of 8.5 to 10; c) refolding the light chain and the solubilized heavy chain of ranibizumab in a second buffer solution comprising 0.1 M arginine to 1 M, 1% sorbitol to 8% and 10 mM Tris to 60 mM having a pH in the range from 10 to 11 and a temperature in the range of 4 to 12 ° C for a first incubation period of 1 to 25 hours and then change the pH and temperature of the first incubation period to a pH in the range of 8 at 9 and a temperature in the range of 20 ° C to 30 ° C for a second incubation period of 1 to 25 hours; and d) recovering said refolded ranibizumab. |
| priorityDate | 2015-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.