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publicationDate 2021-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2841950-T3
titleOfInvention A diagnostic procedure for pancreatic cancer based on lipidomic analysis of a body fluid
abstract A diagnostic procedure for pancreatic cancer based on the lipidomic analysis of a serum obtained from the body of a human patient, characterized in that it comprises the steps of: - enrichment of the sample with a set of internal standards that comprise at least one internal standard for each lipid class present in the sample, - subsequent processing of the sample by liquid-liquid lipidomic extraction, - measurement of the processed sample by a mass spectrometry procedure, - determination of concentrations for at least 60, preferably at least 120 or at minus 230, more preferably for all lipids present at a level above the detection threshold of the mass spectrometry procedure, using the internal standards for the corresponding lipid classes for quantitation, or using relative lipid concentrations, or using ratios of concentrations and / or signal intensities, in which the lipids for the that the concentrations are determined include the following lipids: ** Table ** - statistical evaluation of the determined concentrations of the lipids, said statistical evaluation determining the level of probability that the patient suffers from pancreatic cancer based on the lipid concentrations determined in comparison with a cancerous pattern and a non-cancerous pattern, in which the cancerous pattern and the non-cancerous pattern are determined by statistical analysis of lipid concentrations for a group of known cancer samples and non-cancer samples, in which the statistical evaluation performed separately for men and women.
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