| abstract |
An in vitro or ex vivo method of transducing a target cell, the method comprises the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of approximately 14.6 kDa, wherein the poloxamer is in a fluid state, and wherein said poloxamer has the formula HO (C2H4O) x- (C3H6O) z- (C2H4O) yH, where x + y = approximately 265 and z = approximately 50 on average, where "approximately" refers to to -10% to +20%. |