| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50eb752940fa4aa36756a6bc9f6f3504 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2511-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0682
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
| filingDate |
2014-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61a4ca4ca34f00d5b1cfce6cf903e4c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1dfd6b61073c15e429e05617cacc298
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d246322091a8c9c92a77e2a0e11f3a75 |
| publicationDate |
2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2833471-T3 |
| titleOfInvention |
Metabolically optimized cell culture |
| abstract |
A method of producing a protein of interest comprising: (a) culturing cells comprising a nucleic acid sequence encoding said protein of interest in a first cell culture; (b) determining that a metabolic change to lactate consumption has occurred in said first cell culture; (c) transferring cells from said first cell culture to a second cell culture after said metabolic shift to lactate consumption has occurred; (d) maintaining said second cell culture for a period of time for said protein of interest to accumulate in said second cell culture; (e) harvesting said protein of interest from said second cell culture; and (f) purifying said protein of interest. |
| priorityDate |
2013-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |