http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2833299-T3
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d8960d535ffca17767bf93783fb1d2e6 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6811 |
filingDate | 2015-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c1e02bbf416cacb6076bc4fea09274f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e0ef1e36fa54d66aa9b3d8f1e313e84 |
publicationDate | 2021-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2833299-T3 |
titleOfInvention | Genome fractionation |
abstract | A method of removing an unwanted sequence from a double-stranded nucleic acid library comprising: (a) providing said double-stranded nucleic acid library, comprising a plurality of double-stranded nucleic acid molecules, wherein each double-stranded nucleic acid molecule comprises a first adapter region, a target sequence region, and a second adapter region, and wherein at least one of said double-stranded nucleic acid molecules comprises said unwanted sequence; (b) contacting said double-stranded nucleic acid library with a nucleic acid modifying moiety that specifically binds to said unwanted sequence; wherein said nucleic acid modifying moiety comprises an endonuclease directed by a guide RNA molecule; (c) excising said unwanted sequence so that said first adapter region and said second adapter region are not physically linked through said target sequence region; and (d) sequencing said double-stranded nucleic acid library using a sequencing approach that requires said first adapter region and said second adapter region encompassing said target sequence region. |
priorityDate | 2014-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 612.