| abstract |
Use of genetically modified strains in the preparation of steroidal compounds, characterized in that the genetically modified strains are devoid of the hydroxyacyl-coenzyme A dehydrogenase gene, and the steroidal compounds comprise: 22-hydroxy-23,24-bisorchol-1,4 -dien-3-one, 22-hydroxy-23,24-bisorchol-4-ene-3-one and 9α ,, 22-dihydroxy-23,24-bisnorco1-4-ene-3-one; wherein, the hydroxyacyl-coenzyme A dehydrogenase gene encodes a protein (i) or (ii) as follows: (i) having an amino acid sequence according to SEQ ID NO 2; (ii) which is derived by substitution, deletion or insertion of one or more amino acids in the amino acid sequence defined by (i) and which has the same function as that of the protein of (i). |