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filingDate 2016-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2817000-T3
titleOfInvention Procedure for preparing a topical composition based on dedifferentiated plant cells induced in in vitro culture
abstract Process for preparing a composition for topical use comprising a material derived from plant cells of a species selected from the group consisting of the species of the following families: Agavaceae, Amaryllidaceae, Anacardiaceae, Apiaceae, Apocynaceae, Araceae; Araliaceae, Asclepiadaceae, Asparagaceae, Asphodelaceae, Asteraceae, Balsaminaceae, Begoniaceae, Bombacacea, Brassicaceae, Bromeliaceae, Burseraceae, Cactaceae, Campanulaceae, Capparidaceae, Caricaceae, Chenopodiaceae, Cochlospermaceae, Commelinaceae, Begoniaceae, Bombacacea, Brassicaceae, Bromeliaceae, Burseraceae, Cactaceae, Campanulaceae, Capparidaceae, Caricaceae, Chenopodiaceae, Cochlospermaceae, Commelinaceae, Cvolvulaceae, Convolvulaceae, Cuvolvulaceae, Cuvolvulaceae, Euphorbiaceae, Fabaceae, Fouquieriaceae, Geraniaceae, Gesneriaceae, Hyacinthaceae, Icacinaceae, Lamiaceae, Lentibulariaceae, Loasaceae, Loranthaceae, Melastomataceae, Meliaceae, Menispermaceae, Moraceae, Nolanaceae, Nolinaceae, Orchidaliaceae, Oxalceae, Phulacalanthaceae, Passyldaliaceae, Oxalceae, Passyldaceae, Oxalhaceae, Petallaceae, Oxaldaceae, Rubiaceae, Ruscaceae, Sapindaceae, Saxifragaceae, Sterculiaceae, Urticaceae, Viscaceae, Vitaceae, Xanthorrhoeaceae and Zygophyllaceae, characterized in that dedifferentiated plant cells are placed in an in vitro culture medium to allow the growth of the cells, at the same time as their induction. ion, carrying out said growth and induction in the in vitro medium in a gaseous atmosphere consisting of humid air enriched with CO2 with a content of 1 to 5% by volume of CO2 and a relative humidity of more than 50%, at a temperature between 15 ° C and 50 ° C according to a cycle comprising at least 10 periods of darkness or low luminosity with a luminosity of less than 10 lux for a period of 20 minutes to 3 hours, the periods being separated of darkness or of low luminosity to each other for a period of luminosity in the range of 100 nm to 700 nm, preferably from 400 nm to 520 nm, of more than 1000 lux for a period of 1 to 6 hours, because they are cultured and said dedifferentiated plant cells are induced in their culture medium during this cycle long enough for the synthesis of a sufficient quantity of metabolites in the induced dedifferentiated cells, by which the cells are at least partially separated from the medium of culture, and by mixing the undifferentiated and induced plant cells, not crushed, separated at least partially from the culture medium, with one or more excipients acceptable for topical application in order to prepare the topical composition.
priorityDate 2015-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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