http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2815684-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f9fe21649f0664056e18574118708dc2 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0487 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-525 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2016-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97b3bdfd39fee1cee95ff251655660f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da38f1dd948f735fbffec0cb3b3bed16 |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2815684-T3 |
| titleOfInvention | Improved detection of methylated DNA |
| abstract | A method for detecting a nucleic acid methylation state in an automated system comprising an extraction chamber comprising an extraction membrane, said method comprising the steps of: - introducing a nucleic acid source into the automated system, adding bisulfite thereto and immediately followed by heating the nucleic acid source to produce a lysate comprising the nucleic acid and deamine an unmethylated cytosine base present in said nucleic acid; - adding binding buffer to the lysate to produce a lysate and binding buffer solution; - binding the nucleic acid to the extraction membrane and removing the bisulfite by transporting the lysate solution and binding buffer over the extraction membrane; - add desulfonation buffer to the extraction chamber and transport the desulfonation buffer over the extraction membrane to desulfonate the deaminated base in a previous step in the nucleic acid and convert said base to a uracil base, - elute the converted nucleic acid of the extraction membrane; and analyzing the converted nucleic acid. |
| priorityDate | 2015-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.