http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2811874-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3edb9aa141cda1fdc8cb548d0c57f59c |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1055 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-567 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2014-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24bb103cdc53a827141f6aa40a2b6d74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b2ea9c181659a14019d49ac18215846 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d028651e5f085cf65c575ab63172118f |
| publicationDate | 2021-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2811874-T3 |
| titleOfInvention | Detection of protein-to-protein interactions |
| abstract | A method for quantitatively measuring the strength and affinity of an interaction between a first membrane protein or part thereof and a second membrane or soluble protein or part thereof comprising: (a) providing a host mammalian cell including a detectable gene (reporter gene) having a binding site for a transcription factor, such that the detectable gene expresses a measurable detectable product when the detectable gene is transcriptionally activated; (b) expressing in the host mammalian cell the first protein or part thereof, the first protein or part thereof being linked via a suitable first linker to a C-terminal subdomain of a human ubiquitin (Cub) and the transcription factor; (c) expressing in the host mammalian cell the second protein or part thereof, the second protein or part thereof being linked to a wild-type N-terminal subdomain of the human ubiquitin protein (Nub) through a suitable second linker; and (d) measuring a detectable product expression output as a measure of the amount of interaction between the first and second proteins, wherein the first linker and the second linker is a peptide comprising the amino acid sequence (GGGGS)n , wherein "n" is an integer equal to or greater than 1, and wherein the detectable gene includes a fluorescent reporter gene and the detectable product is luciferase. |
| priorityDate | 2013-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 223.