abstract |
A method of producing a fatty acid or derivative thereof, comprising; (a) providing an engineered bacterial host cell comprising a heterologous promoter and / or ribosome binding site operably linked to a polynucleotide sequence encoding a FadR polypeptide, causing said promoter and / or ribosome binding site overexpression of the FadR polypeptide in said cell, (b) culturing the genetically engineered bacterial host cell in a culture medium under conditions that allow the production of a fatty acid or a derivative thereof, and (c) isolating the fatty acid or derived thereof from the genetically engineered bacterial host cell, wherein the fatty acid or derivative thereof is a fatty acid, an acyl-ACP, an acyl-CoA, a fatty aldehyde, a short-chain alcohol, a long chain alcohol, a fatty alcohol, a hydrocarbon, or an ester. |