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grantDate 2021-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2021-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2805794-T3
titleOfInvention Photocoupling procedure using a probe containing photosensitive nucleic acids
abstract A photocoupling method, characterized by hybridizing to a target site present in a nucleic acid sample with a first probe having a sequence complementary to the target site and containing a photosensitive nucleotide, in a reaction solution, and carrying out photocoupling by photoirradiation, wherein the self-assembly caused by the photosensitive nucleotide contained in the first probe is suppressed by coexisting with a second probe that is highly complementary to the first probe, and wherein the first probe and the second probe are designed such that the photosensitive nucleotide in the first probe is not photocoupled with the second probe, where being highly complementary means a state in which the first probe and the second probe are complementary to each other, and a base in the first probe that photocouples with the photosensitive nucleotide under predetermined photocoupling conditions hybridizes to the second probe.
priorityDate 2012-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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