http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2799431-T3
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b5614fff8cd42df639351e4287bf2ba4 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
filingDate | 2011-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61a40c7f3d512b3a1539a1564a68870f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7347926370fa9446d8cdb069ce1d24de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c95261ebde348ac7cfa260b0d2cf524 |
publicationDate | 2020-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2799431-T3 |
titleOfInvention | Insoluble target protein separation |
abstract | A method of isolating an insoluble target silk protein from a suspension of intact or disrupted host cells comprising the steps of: a) providing a suspension of intact or disrupted host cells comprising an insoluble target silk protein and insoluble cell parts host, b) adding an aqueous solution of at least one base to said suspension, wherein the base is in an amount that is sufficient to disrupt said host cells and/or solubilize said insoluble parts of the host cell, and c) separating the insoluble target silk protein from solubilized host cell insoluble portions, wherein in step b) the target silk protein remains insoluble, and wherein at least 80% of the host cell insoluble portions are solubilized . |
priorityDate | 2010-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.