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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14
filingDate 2011-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61a40c7f3d512b3a1539a1564a68870f
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publicationDate 2020-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2799431-T3
titleOfInvention Insoluble target protein separation
abstract A method of isolating an insoluble target silk protein from a suspension of intact or disrupted host cells comprising the steps of: a) providing a suspension of intact or disrupted host cells comprising an insoluble target silk protein and insoluble cell parts host, b) adding an aqueous solution of at least one base to said suspension, wherein the base is in an amount that is sufficient to disrupt said host cells and/or solubilize said insoluble parts of the host cell, and c) separating the insoluble target silk protein from solubilized host cell insoluble portions, wherein in step b) the target silk protein remains insoluble, and wherein at least 80% of the host cell insoluble portions are solubilized .
priorityDate 2010-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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