http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2798271-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74936e49a77ba0928def46130724cfb8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-027 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D71-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2016-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bca053a8addc4429d94c913c2fb0ba8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c65d9c47de8c3914c97cdb036c9558aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_277fd4baaca517a513bd50fa8ddd3136 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a570e75d7c2a396e4202c0ba6cc47fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_556b4cd3ef01a6717b5399419eece82a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d89590f8de61936903bc7bf132bd2a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4322298548a1ed2ec2f04c908569a16 |
| publicationDate | 2020-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2798271-T3 |
| titleOfInvention | Method for producing and purifying RNA, comprising at least one tangential flow filtration step |
| abstract | A method for producing and purifying RNA, comprising the steps of A) providing DNA encoding the RNA; B) performing in vitro transcription of DNA to produce a solution comprising transcribed RNA; and C) carrying out the diafiltration and / or concentration and / or purification of the solution comprising the transcribed RNA by means of one or more tangential flow filtration (TFF) steps, wherein the method comprises in step C) the steps of : C1) optionally perform transcription termination; C2) carry out the diafiltration and / or concentration and / or purification of the solution comprising the transcribed RNA by means of one or more TFF steps; C3) carry out the purification of the RNA by means of an additional purification method, in which said additional purification method does not comprise a step of extraction with phenol / chloroform and / or precipitation of DNA and / or RNA, in which the purification method additional is done by means of reverse phase HPLC; and C4) carry out the diafiltration and / or concentration and / or purification of the solution comprising the transcribed RNA obtained after step C3) by one or more TFF steps, wherein the at least one or more TFF steps comprise use a TFF membrane cassette. |
| priorityDate | 2015-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 311.