http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2794401-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7357bedd6aa8ef1bf76550214db257d4 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
| filingDate | 2009-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c543e2098a5b568966e76da81c0c0ee5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_88fc724cf0c6319393b82d793afaee3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ddbadfd116bb491895f345d4a5de3f9 |
| publicationDate | 2020-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2794401-T3 |
| titleOfInvention | Oligonucleotide detection method |
| abstract | A method for detecting an oligonucleotide and its oligonucleotide metabolites, wherein the oligonucleotide is a single-stranded oligomer of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA), comprising the steps of (a) selecting a biological sample containing or is suspected of containing said RNA oligonucleotide, in which the sample is tissue or plasma lysate, (b) forming a hybridization mixture by contacting the biological sample with a fluorescently labeled peptide nucleic acid (PNA) probe that is fully complementary to at least or more nucleotides of said oligonucleotide, (c) separating hybridized residues formed between said oligonucleotide and said PNA probe from non-hybridized residues by means of high performance anion exchange liquid chromatography (HPLC) characterized in that the excess of the non-hybridized PNA probe is eluted in the initial volume of the HPLC, and (d) quantitatively detect said hybridized residues s by fluorescence spectroscopy, further characterized in that the oligonucleotide metabolite signals are separated from the signal generated by the intact oligonucleotide. |
| priorityDate | 2008-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.