http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2791328-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4cfa17f7e32f39006b7d3d116b52b41d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_931e133028ed3a2b24a46de06eb44919 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f417b1296c2a31ff393881065bf7484 |
| publicationDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2791328-T3 |
| titleOfInvention | Acellular DNA quantification methods |
| abstract | A method of quantifying chimeric NacDNA in a sample from a patient, the method comprising: (a) providing an acellular DNA (cDNA) sample from a blood, serum or plasma sample obtained from a patient; (b) performing a multiplex digital PCR comprising at least a first amplification and a second amplification, wherein the first amplification targets a first single-copy genomic locus and results in the production of a first amplicon and a second amplification targets a second single copy genomic locus and results in the production of a second amplicon, where the first and second amplicons differ in length by at least 50 base pairs; (c) determining the proportion of the first or second amplicon in the total amplified product from the digital PCR of step (b) to provide a correction factor for the amplifiable fraction of the cDNA sample, and (d) correcting a Diagnostic digital PCR performed on the cDNA sample to evaluate the level of chimeric DNA present in the sample, using the correction factor determined in step (c). |
| priorityDate | 2014-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 116.