http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2776873-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_134e761e8dcbff4985fa1e77a9c2062e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T2207-20076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T2207-30096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T2207-10056 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T2207-30024 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T7-0012 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T7-0014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06V20-69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-57484 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06T7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06K9-00 |
| filingDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_660ab3e373e8980c8582d8df6ed16231 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f287b8b7b74a249a74b950e7419b4985 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4947db63fd338713e09b4118a88ecf72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ccd917ce1b510bb532fefe3a62239ce |
| publicationDate | 2020-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2776873-T3 |
| titleOfInvention | Procedure for quantitative measurement of a biomarker by in situ immunofluorescence and its uses |
| abstract | A method for the quantitative measurement of a target marker by in situ immunofluorescence comprising the steps of: a) supplying an immobilized sample on a sample holder; b) performing a staining step that comprises incubating said sample with at least one target probe, at least one screening probe to obtain images of the sample region of interest (first screening probe) and, optionally, by minus one additional screening probe for the sample region of no interest to be discarded in a subsequent analysis (second screening probe), where the incubation times of the sample with each of said probes are low enough to avoid saturation of the sample with the probes, while ensuring adequate staining of the sample so that a linear relationship can be formed between the concentration of the target marker and the intensity of the resulting fluorescent signal, where the incubation times of the sample with each of said probes are less than 16 minutes; c) acquiring an image of the stained sample comprising raw signals emitted by each of the imaging probes; d) generating a target evaluation mask comprising an active area for the analysis of said target marker, comprising defining a threshold for the signals of said at least one screening probe, and assigning binary values to the screening probe signals, said binary values comprising a first value (for example, 0) and a second value (for example, 1), whereby screening probe signal values that are beyond said corresponding threshold are set at said first value, and screening probe signal values that are not beyond said corresponding threshold are set at said second value, said active area of the mask being defined by areas of the image comprising signals set at said second value; e) applying the active area of the target evaluation mask over the raw signal values of the first screening probe to obtain a set of screening probe data comprising values proportional to the raw signal values of the first screening probe for active area; f) processing the target probe signals, including extracting target probe signal data limited to said active area to obtain a target probe data set comprising target probe signal values for the active area; and g) generating a sample output that includes combining said target probe data set with said screening probe data set to provide information on quantitative levels of the target marker. |
| priorityDate | 2016-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.