abstract |
A recombinant factor VIII fusion protein comprising a factor VIII polypeptide and at least one extended recombinant polypeptide (XTEN), wherein the factor VIII polypeptide comprises an A3 domain, an A2 domain, an A1 domain, a C2 domain , a C1 domain and a B domain or a part of the B domain, in which the at least one XTEN is inserted into the factor VIII polypeptide at a site corresponding to amino acids 745 of the sequence set forth in SEQ ID NO: 1592 , wherein the recombinant FVIII fusion protein retains at least 10% of the procoagulant activity of a corresponding factor VIII polypeptide lacking said XTEN, and wherein the recombinant FVIII fusion protein exhibits a prolonged terminal half-life when administered to a subject in comparison to a corresponding factor VIII polypeptide grown from said XTEN. |