http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2769796-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_54ae5e452ed609179e635c91ad116e6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6593c15d7a40419ed1de224db1dea4c3 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2013-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6f1fe546a9c937fb976c5a647cac394 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7af6bac664225cec55107223ba8d8b44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ddddd8c10a1d59c8759785af5def9a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f09b7ecafb24e66cf2f29587cc24cbf1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da455be1ed822e2a31a3dc106976ef73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_979c81bb6f6387b94d91852872cf7ea4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98dcd73cd420455d9890692618ae444d |
| publicationDate | 2020-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2769796-T3 |
| titleOfInvention | Increased blocking oligonucleotides in Tm and decoys for improved target enrichment and reduced off-target selection |
| abstract | Method of selecting a desired template nucleic acid from a template nucleic acid population, comprising: (a) contacting the template nucleic acid population with a first oligonucleotide comprising a Tm-increased oligonucleotide as a blocker to form a mix; and (b) isolating the desired template nucleic acid from the mixture, wherein the step of contacting the population of template nucleic acids with a first oligonucleotide comprising a Tm-increased oligonucleotide comprises incubating the mixture at a temperature that presents a Tm value of the oligonucleotide increased by Tm, wherein the step of isolating the desired template nucleic acid comprises: (i) forming a hybrid complex between the desired nucleic acid and a second oligonucleotide as a decoy; and (ii) separating the hybrid complex from the mixture in which the Tm-augmented oligonucleotide comprises a plurality of residue groups selected from a group consisting of a group of blocked nucleic acids, a group of bicyclic nucleic acids, a pyrimidine. modified in C5, a group of peptide nucleic acids or combinations thereof, the oligonucleotide increased in Tm is complementary to a non-target nucleic acid and forms a duplex with said non-target nucleic acid sequence selected from adapter and code sequences of bars, and the step of contacting the template nucleic acid population with a first oligonucleotide comprising a Tm-increased oligonucleotide results in inhibition of complex formation between the desired template nucleic acid and the unwanted template nucleic acids. |
| priorityDate | 2012-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 258.