http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2763822-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf32b60391b469c41e86c353437f5751 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6813 |
| filingDate | 2016-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5d8e873947548f35223ab4c2b68829b |
| publicationDate | 2020-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2763822-T3 |
| titleOfInvention | Mark-free analysis of the efficiency of adding caps to the RNA using rnasa h, probes and liquid chromatography / mass spectrometry |
| abstract | A radiolabel-free method for identifying a 5 'end cap on a target ribonucleic acid (RNA), comprising the steps of: (a) hybridizing a non-radiolabeled labeled probe to the target RNA, where the nucleotide sequence of the probe labeled non-radiolabeled is complementary to the 5 'end of the target RNA, thereby forming a duplex polynucleotide; (b) treating the duplex polynucleotide with RNase H, thereby cleaving the 5 'end of the target RNA and forming a duplex polynucleotide containing the 5' end of the target RNA; (c) isolating the duplex polynucleotide, using a surface-coated substrate that is coated with a reagent that binds to the non-radiolabeled labeled probe; (d) removing the duplex polynucleotide from the coated surface substrate; (e) denaturing the duplex polynucleotide, thereby producing a single-stranded fragment of the 5 'end of the target RNA and the non-radiolabeled labeled probe; (f) isolating the single stranded fragment from the 5 'end of the target RNA; (g) analyzing the single stranded fragment from the 5 'end of the target RNA by liquid chromatography / mass spectrometry (LC-MS); and (h) identifying the 5 'end cap. |
| priorityDate | 2015-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 268.