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filingDate 2012-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e13c16b9e43cacf6b95d53632144687a
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publicationDate 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2763089-T3
titleOfInvention Agent for the measurement of endotoxins
abstract Process for producing a horseshoe crab factor C comprising an amino acid sequence shown in SEQ ID No .: 2 or having an identity of not less than 90% of the total length of the amino acid sequence shown in SEQ ID No .: 2 and having factor C activity, which is a recombinant protein that does not have a His tag sequence at the C-terminal end or any other peptide at any terminal end and is obtained from cells of insect as host: the method comprising the following steps (A) to (D): (A) a step of incorporating DNA encoding the amino acid sequence of factor C into a viral DNA; (B) a step of infecting insect cells with the virus into which said DNA has been incorporated; (C) a step of allowing insect cells infected with said virus to express the protein encoded by said DNA; and (D) a step of recovering factor C as a solution and removing the virus using a hollow fiber filtration membrane having a pore size of 500 kDa.
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type http://data.epo.org/linked-data/def/patent/Publication

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