http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2760508-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50eb752940fa4aa36756a6bc9f6f3504 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b0e65f6722dab22c8233aa56f25f2bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_973ff7ce33b9c5f730a90445e1a94cfb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c8d1cd98c9443bef55857b995fdc4e67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_002a39fc88e47fdb3330af4094f5b722 |
| publicationDate | 2020-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2760508-T3 |
| titleOfInvention | Methods and compositions for targeted genetic modification through single-stage multiple transformation |
| abstract | A method of modifying a target genomic locus in a mammalian cell, comprising: (a) introducing into the cell a nuclease-like agent that produces a single or double-stranded break within the target genomic locus; (b) introducing into the cell a first large transformation vector (LTVEC) that is at least 10 kb in length and comprises a first nucleic acid insert flanked by a first 5 'homology arm and a first 3' homology arm , and a second LTVEC that is at least 10 kb in length and comprises a second nucleic acid insert flanked by a second 5 'homology arm and a second 3' homology arm, wherein the combined size of the first acid insert nucleic acid and the second nucleic acid insert is at least 100 kb, wherein the first 3 'homology arm of the first LTVEC has a first overlapping sequence homologous to the second 5' homology arm of the second LTVEC, and the first arm of 5 'homology of the first LTVEC and the second 3' homology arm of the second LTVEC are homologous to the corresponding genomic segments within the target genomic locus, where the first overlapping sequence is at least 1 kb, and where e the target genomic locus is modified by integrating the first nucleic acid insert and the second nucleic acid insert between the corresponding genomic segments; and (c) selecting a transformed cell that comprises the first nucleic acid insert and the second nucleic acid insert integrated into the target genomic locus. |
| priorityDate | 2014-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1204.