abstract |
A method of modifying a protein, comprising: providing a target protein having at least one lysine residue; contacting the target protein with a modifier compound having the formula R1- (Leu) x-Gln- (Gly) and- (A-W-B-R2) z in the presence of a microbial transglutaminase to form a modified protein; where x is 0 or 1; y is 0 or 1; z is 0 or 1; R1 is ** Formula ** W is selected from: linear or branched C1-C6 alkyl or polyethylene glycol having a molecular weight of between about 40 and about 80,000 amu; A is absent or is selected from -O-, -NH- and -S-; B is absent or selected from -O-, -C (O) -, -NH-, -C (O) NH-, -NHC (O) -, -NHC (O) O-, -OC (O) NH-, - OC (O) O-, -C = N (OH) -, -S (O2) -, -NHS (O2) -, -S (O2) NH-, -S (O) -, - NHS (O) -, -S (O) NH-; -C (O) O-, -OC (O) -, -S-, = NH-O-, = NH-NH- and = NH-N (C1-C20 alkyl) -; R2 is selected from the group consisting of: a linear or branched (C1-C3 alkyl) -N3 fatty acid, cyclooctinyl, polysaccharide, -CH (OCH3) 2, ** Formula ** each n is an integer independently selected from 0 to 6; each Q is selected from H and NO2. |