http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2754785-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e704ea18b7d505b1ff94ce67a0d88ebb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
| filingDate | 2017-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e4fbec0b9319065ee94e8cb08b9c16d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2872cc79a35ec0806061b0907553498 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_558b6ca9fe87debe552b4b6d2168c165 |
| publicationDate | 2020-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2754785-T3 |
| titleOfInvention | DNA repair results modulation procedures |
| abstract | A method of predictably inserting a single nucleotide into a target region at a target site in DNA after Cas9-mediated cleavage, the method comprising: selecting a gene comprising the target region to be modified; designing a guide polynucleotide to target a selected 20 nt protospacer in the target region; producing a double-stranded break at the target site in the target region using a Cas9 protein and the guide polynucleotide complementary to the protospacer in the target region such that the nucleotide at position 17 of the protospacer corresponds to an A or a T; wherein (i) for the insertion of an A at the double-stranded break at the target site, position 17 of the protospacer is A, or (ii) for the insertion of a T at the double-stranded break at the site target, position 17 of the protospacer is T; thus predictably inserting a single nucleotide into the target site after Cas9-mediated cleavage; wherein the method does not modify the genetic identity of the germ line of a human; wherein the method is not a method for treating the human or animal body by therapy. |
| priorityDate | 2016-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.