| abstract |
A process for the production of an attenuated RNA virus or an attenuated cDNA clone thereof, by attenuating an infectious RNA virus or an infectious cDNA clone thereof, wherein said infectious RNA virus or cDNA clone Infectious disease thereof comprises an RNA-dependent RNA polymerase, wherein said process comprises providing an infectious RNA virus or an infectious cDNA clone thereof, wherein said infectious cDNA clone comprises the cDNA sequence, which is the reverse transcription of the CDS of the genome of said RNA virus, modifying the RNA genome of said infectious RNA virus or the retrotranscribed cDNA sequence of said cDNA clone, respectively, wherein said modification comprises replacing at least one codon, encoding Leu, Ser, Arg or Gly in said infectious RNA virus or infectious cDNA clone, by a different but synonymous codon, wherein said different but synonymous codon is a codon, which differs by just one nuc leotid of a STOP codon. |