| abstract |
A protein comprising: a Fab arm that binds to a first epitope, a binding domain (BD) that binds to a second epitope, and an Fc region that comprises CH2 and CH3 domains; wherein the protein comprises a chimeric heavy chain comprising the following polypeptide domains, from the N-terminus to the C-terminus VH1-CH1-L1-BD-L2-Fc-L2-BD-L1-CH1-VH1; wherein VH1 comprises the Fab heavy chain variable domain, CH1 comprises the Fab heavy chain 1 constant domain, L1 comprises the first linker polypeptide, BD comprises a scFv, L2 comprises the second linker polypeptide and Fc comprises a CH2 and a CH3 domain and wherein the protein is bivalent to bind to each of the first and second epitopes, and wherein L1 comprises the amino acid sequence EPKSCDKT (SEQ ID NO: 4) or EPKSCGKT (SEQ ID NO: 6) or EPKSC (SEQ ID NO: 38) and / or wherein L2 comprises the amino acid sequence EPKSVDKTHTCPPCP (SEQ ID NO: 12) or DKTHTCPPCP (SEQ ID NO: 40). |