http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2743512-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_441be42e74acc2c8056db556e92a73ca |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8239 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8243 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2015-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2819955bc4218037ddf2bf29c223f8dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4039aa6a0ae853f6d43c5ceb76d2080c |
| publicationDate | 2020-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2743512-T3 |
| titleOfInvention | Synthetic promoter library for coordinated gene expression in eukaryotic cells or organisms |
| abstract | A method of expressing two or more nucleic acid sequences of interest in eukaryotic cells, in cells or tissues of a eukaryotic organism, or in a eukaryotic organism excluding humans, comprising: (a) providing eukaryotic cells, tissue from a eukaryotic organism or a eukaryotic organism excluding humans comprising two or more nucleic acid constructs of interest, each nucleic acid construct comprising a promoter and, downstream thereof, a nucleic acid sequence of interest to be expressed, wherein each promoter of said nucleic acid constructs of interest are selected from a library of multiple promoters each comprising, in the 5 'to 3' direction, (i) an effector binding site comprising at least 10 contiguous bases, wherein the sequences base of the effector binding sites of multiple promoters have a sequence identity of at least 7 5%, (ii) a TATA box, and (iii) a downstream sequence segment comprising at least 20 contiguous bases, where the downstream sequence segments of at least two nucleic acid constructs of interest differ, and ( b) providing the eukaryotic cells, the tissue of a eukaryotic organism or a eukaryotic organism of the previous stage with an effector capable of binding to the effector binding sites of the promoters and activating the transcription of said promoters. |
| priorityDate | 2015-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 134.