| abstract |
A method of producing at least two different antibodies from a single host cell, wherein each of the two antibodies comprises two CH3 domains that are capable of forming an interface, the method comprises providing in said cell a. A first nucleic acid molecule encoding a 1st polypeptide chain comprising the CH3 domain of human IgG comprising a lysine (K) residue at amino acid position 366 according to the EU numbering system, b. A second nucleic acid molecule encoding a 2nd polypeptide chain comprising the CH3 domain of human IgG comprising an aspartic acid residue (D) at amino acid position 351 according to the EU numbering system, c. A third nucleic acid molecule encoding a 3rd polypeptide chain comprising the CH3 domain, and d. A fourth nucleic acid molecule encoding a 4th polypeptide chain comprising the CH3 domain, the method further comprises the step of cultivating said host cell and allowing the expression of said at least four nucleic acid molecules and harvesting said at least two different antibodies from the culture. |