| abstract |
A bispecific antibody fusion protein that binds human OX40 and human serum albumin, and antagonizes the binding of OX40 to OX40L, the bispecific antibody fusion protein comprising: a heavy chain comprising, in sequence from the N-terminus, a first heavy chain variable domain (VH1), a CH1 domain and a second heavy chain variable domain (VH2), a light chain comprising, in sequence from the N-terminus, a first light chain variable domain (VL1), a domain CL and a second light chain variable domain (VL2), in which said heavy and light chain are aligned so that VH1 and VL1 form a first antigen binding site and VH2 and VL2 form a second antigen binding site, wherein the antigen bound by the first antigen binding site is human OX40 and the antigen bound by the second antigen binding site is human serum albumin, wherein the first heavy chain variable domain (VH1) comprises nde the sequence given in SEQ ID NO: 1 for CDR-H1, the sequence given in SEQ ID NO: 2 for CDR-H2 and the sequence given in SEQ ID NO: 3 for CDR-H3 and the first variable domain of the light chain (VL1) comprises the sequence given in SEQ ID NO: 4 for CDR-L1, the sequence given in SEQ ID NO: 5 for CDR-L2 and the sequence given in SEQ ID NO: 6 for CDR -L3, in which the second heavy chain variable domain (VH2) has the sequence given in SEQ ID NO: 11 and the second light chain variable domain (VL2) has the sequence given in SEQ ID NO: 12 and the second heavy chain variable domain (VH2) and the second light chain variable domain (VL2) are linked by a disulfide bond. |