http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2730980-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_589ee83a8f94a6e7bd6ecb740533b7b6 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2012-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa47b95138e4d2febb3b15d17de38db1 |
| publicationDate | 2019-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2730980-T3 |
| titleOfInvention | Method of evaluation of immunodiversity and its use |
| abstract | A method for assessing the level of diversity of an immunorepertory comprising the steps of: (a) amplifying polynucleotides from a population of white blood cells from a human or animal subject in a reaction mixture comprising target-specific nested primers to produce a set of first amplicons, at least a portion of the target-specific nested primers comprising additional nucleotides that, during amplification, serve as a template to incorporate into the first amplicons a binding site for at least one common primer; (b) transferring a portion of the first reaction mixture containing the first amplicons to a second reaction mixture comprising at least one common primer; (c) amplifying, using at least one common primer, the first amplicons to produce a set of second amplicons; (d) sequencing the second amplicons to identify V (D) J rearrangement sequences in the white blood cell subpopulation, (e) use the identified V (D) J rearrangement sequences to quantify both the total number of cells in a immune system cell population as the total number of cells within each distinct CDR3 clonotype identified within the population; and (f) identify the number of distinct CDR3 clonotypes that comprise a significant percentage (N) of a total number of cells counted within that population, in which a normal state is characterized by the presence of a greater variety of CDR3 clonotypes. represented within the significant percentage (N) of the total number of cells and an abnormal state is characterized by the presence of a smaller number of different CDR3 clonotypes represented within the significant percentage (N) of the total number of cells, in which the diversity of the different CDR3 clonotypes is measured by the diversity index (DN), in which DN is determined by C / S x 100, in which C is a minimum number of different CDR3 clonotypes that represent a percentage greater than or equal to N of a total of sequencing reads obtained after amplification and sequencing of the polynucleotides isolated from the population of cells in which S is the number of clonotypes of CDR3 di stintos in classified dominance configuration. |
| priorityDate | 2011-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.