http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2715952-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9b7e3799d02776160735934b621d0747 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-885 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12C1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L2-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12C7-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12C5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
| filingDate | 2010-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56dd895b571ded902c0c7fd31c7455e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_696bfdb9b9f801278ee209d62ab14d76 |
| publicationDate | 2019-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2715952-T3 |
| titleOfInvention | Enzymatic enzyme complex of Trichoderma Reesei and P. Funiculosum |
| abstract | An enzyme complex having beta-1,4-endoglucan hydrolase activity comprising: a. a fermentation product of Trichoderma reesei; and b. a Penicillium funiculosum fermentation product comprising one or more enzymes selected from a xylanase (EC 3.2.1.8), a cellulase (EC 3.2.1.4) and a beta-glucanase (EC 3.2.1.6); where the ratio of the beta-1,4-endoglucan hydrolase activity of Penicillium funiculosum and Trichoderma reesei is from 0.25 / 0.75 to 0.35 / 0.65 and where the beta-1,4- Endoglucan hydrolase of the enzyme complex is measured using the following assay: a unit of cellulase activity is defined as the amount of enzyme that produces 1 mmol glucose equivalents per minute under the conditions of the assay (pH 5.0) and 50 ° C; The enzyme complex is diluted in samples and a glucose standard curve is made using glucose concentrations of 0, 0.125, 0.25, 0.375, and 0.5 mg / ml; 0.25 ml of enzyme solution is mixed with 1.75 ml of substrate solution (carboxymethylcellulose (CMC) solution at 1.5% w / v in 0.1M sodium acetate buffer, pH 5.0) at 50 ° C and the reaction is stopped after 10 min by adding a 3,5-dinitrosalicylic acid (DNS) solution (20 g / l of DNS in buffer containing 32 g / l of sodium hydroxide pellets and 600 g / l sodium and potassium tartrate (+)). This is followed by heating at 95 ° C for 5 minutes; Optical density is measured at 540 nm (OD540 nm). Enzyme activity is determined from the standard curve. The activity is calculated as follows: |
| priorityDate | 2009-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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Total number of triples: 120.