http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2713526-T3

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cbffa3b81b3b862dc7220b5648f5f745
classificationCPCAdditional http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10
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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
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filingDate 2010-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54dd740f0b8cb4a97def0f3079fd5e21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d91740b20224b5f09dd8fbf39d70746
publicationDate 2019-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2713526-T3
titleOfInvention Process for the production of alkyl esters of fatty acids using microorganisms that have the capacity to produce oil
abstract A method of producing an alkyl fatty acid ester using Rhodococcus opacus, the process comprising the steps of: (a) cultivating Rhodococcus opacus that has the ability to produce oil and transformed with a first gene encoding a triacylglycerol lipase and a second gene which encodes a monoacrylic glycerol lipase, thus producing oil; (b) induce the autolysis of the oil produced in Rhodococcus opacus to produce a free fatty acid, in which the autolysis is carried out in Rhodococcus opacus by triacyl glycerol lipase and monoacyl glycerol lipase; and (c) adding an alcohol to the free fatty acid produced and reacting the alcohol with the free fatty acid to produce an alkyl fatty acid ester, wherein the first gene comprises SEQ ID NO: 19, and the second gene comprises the M. smegmatis MAG lipase gene produced by PCR amplification of M. smegmatis genomic DNA with the primers of SEQ ID NO: 13 and SEQ ID NO: 14 according to Example 2.
priorityDate 2009-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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