http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2708561-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eec40d1e34ae619a1a28c526b18c6174 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1017 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2014-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bdeef1bc5ee28674a960b14e8dfabda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9e0695144ca7567391bfa4eb7328d58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcb4b7d6b5be3e1b563811d0c7c8c92c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d63fb5231e0bafd90dfafbd3291b0e46 |
| publicationDate | 2019-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2708561-T3 |
| titleOfInvention | Methods for the purification of messenger RNA |
| abstract | A method of purification of messenger RNA (mRNA), comprising (a) synthesizing mRNA by In Vitro transcription using the following reagents: (i) a linear or circular DNA template containing a promoter; (ii) a set of ribonucleoside triphosphates; (iii) a buffer system that optionally includes DTT and magnesium ions; and (iv) an RNA polymerase; and optionally (v) a DNase I, a pyrophosphatase and / or an RNAse inhibitor; (b) adding a protein denaturing agent to the preparation of step (a), which comprises the In Vitro synthesized mRNA, wherein the protein denaturing agent is a chaotropic agent or a high salt concentration; and (c) purifying the mRNA from the preparation of step (b) by tangential flow filtration, wherein the tangential flow filtration comprises: (i) a transmembrane pressure between 1 and 30 pounds per square inch; (ii) a feed rate between 50 and 500 mL / minute; (iii) a flow rate between 10 and 100 mL / minute; and (iv) a filter having a nominal molecular weight limit of between 200 kDa and 700 kDa, wherein the purified mRNA of step (c) contains undetectable levels of prematurely aborted RNA sequences and enzymatic reagents used in In Vitro synthesis as determined by agarose gel electrophoresis or chromatographic methods. |
| priorityDate | 2013-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 263.