| abstract |
An in vitro method for culturing human stem cells without the need for feeder cells or conditioned medium, the method including the step of culturing human stem cells in a culture medium comprising, in amounts sufficient to maintain the stem cells in a undifferentiated state across multiple culture passages, albumin, minerals, vitamins, amino acids, glucose, lipids, a transferrin or a transferrin substitute, insulin or an insulin substitute and all but one of the following constituents: fibroblast growth factor in a concentration of 10 ng / ml to 1000 ng / ml, gamma amino butyric acid, pipecolic acid, TGFß and a lithium salt, the medium being free of feeder cells and never having been exposed to feeder cells. |