| abstract |
A method for the collection and subsequent passage of human pluripotent stem cells without scraping and without substantial loss of viability, comprising: incubating said human pluripotent stem cells in a formulation, said formulation comprising: sodium citrate, and a salt, selecting the salt between the group consisting of NaCl, KCl, Na2HPO3, NAH2PO3, K2HPO3, KH2PO3 and NaHCO3, said formulation having an osmolarity of 150-1,500 mOsmol / liter. |