| abstract |
Method of recovery and / or purification of a recombinantly expressed intracellular protein comprising the annexin sequence A5 (AnxA5) of an endotoxin-producing host cell with a cell wall, the method comprising releasing the intracellular protein from the host cell , characterized in that the step of releasing the intracellular AnxA5 protein is carried out in the presence of a homogenization buffer comprising a non-ionic detergent, and preferably in which the method does not include any centrifugation step for the recovery and / or purification of the AnxA5 protein after its release from the host cell and / or in which the AnxA5 protein remains in solution throughout the procedure, except when it is temporarily bound to some chromatographic resin. |