http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2694349-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_766802ff2e94615e2c615daf957853d5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 |
| filingDate | 2014-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2283bd25542e21111130813c0c1dcf6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f06e444eb724c2986ffa9837fbaa8b2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f99981f5c69617324fa02a40497e4e0e |
| publicationDate | 2018-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2694349-T3 |
| titleOfInvention | Novel system of alteration of genomes for microorganisms |
| abstract | A group of targeting constructs, comprising a first construct comprising, in this order, a first region of homology with a target genome of a microorganism, a recognition site for an endonuclease, and a first part of a selection marker and a second construction comprising, in this order, a second part of the selection marker, a copy of the endonuclease recognition site and a second region of homology with the target genome of the microorganism, whereby the first and second regions of homology with the target genome each comprises at least 20 base pairs (bp); a fragment of the first part of the selection marker overlaps with a fragment that is present in the second part of the selection marker, allowing recombination between the first and second part of the selection marker, said fragment preferably comprising between 50 and 600 bp; a coding sequence that encodes the endonuclease and that is coupled to an inducible promoter is present in the first or second construct; and a portion of the first region of homology to the target genome in the first construct is duplicated between the copy of the endonuclease recognition site and the second region of homology to the target genome in the second construct; or a part of the second region of homology with the target genome in the second construct is duplicated between the first region of homology with the target genome and the endonuclease recognition site in the first construct, said duplicated region comprising between 20 and 200 bp . |
| priorityDate | 2013-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1499.