http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2694285-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cfa8fbae9138511e541b60be72de519b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2016-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0fbd4eebd7e4b24d10ec1d4abab99fff |
| publicationDate | 2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2694285-T3 |
| titleOfInvention | Device and procedure for the isolation of nucleic acids from whole blood |
| abstract | Use of a composition as a disintegrating agent for cells contained in whole blood, as a stabilizing agent for whole RNA from whole blood and for isolation by adsorption of RNA from a mixture of whole blood with the composition to an adsorption agent for nucleic acids, characterized in that the aqueous solution composition is composed of a. at least one guanidinium salt, b. at least one buffer substance for buffering at a pH of 5.0 to 8.0, c. at least one nonionic detergent and d. at least one complexer for divalent cations, e. and, optionally, proteinase and / or DNase, and f. it is free of reducing agents, which prevents the degradation and renewed synthesis of RNA in whole blood, and because the use comprises the isolation of RNA directly from the mixture, which is composed of whole blood and the composition, being provided the composition in a variable volume ratio of whole blood to the composition of up to 1:13 in the mixture, by contacting the mixture with an adsorption agent for nucleic acids, where from the mixture there is no Separate any component, optionally to the mixture is added proteinase and / or DNase, and the RNA does not precipitate from the mixture and separates before it is contacted with the adsorption agent. |
| priorityDate | 2016-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 253.