| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec0024795f45a646ef64b3664ab8a26d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14352
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14344
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14343
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14351
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10344 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-864
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-015
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 |
| filingDate |
2011-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b00261317e4594b6474e06a489469142
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b786ca2d27ffbfd0c356dc5c66efd224
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ead879f6dacdc19789a57a1f534900d5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a00b6af64d15812fb3236604f20f911b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc7a3da4986ced62493a339289d9e671 |
| publicationDate |
2018-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
ES-2683002-T3 |
| titleOfInvention |
Effective production of recombinant autonomous parvoviruses using a helper vector obtained from adenovirus |
| abstract |
A method of preparing a recombinant autonomous parvovirus characterized in that (i) HEK 293T cells are transfected with (a) a recombinant parvovirus vector obtained from the rat parvovirus H-1 (H-1) or the tiny mouse virus ( MVM) that lacks the genes of the virus VP1 and VP2 capsid proteins but that conserves the coding sequences of the nonstructural protein NS1 / 2, b) a vector that contains the genes that encode the VP1 capsid proteins and Parvovirus VP2, and (c) a helper vector obtained from adenovirus comprising the Ad5 adenoviral DNA sequences of the E2a gene, the E4 gene (orf6) and a VAI RNA gene; and (ii) the recombinant parvovirus lacking the VP1 and VP2 genes but retaining the NS1 / 2 coding sequences is isolated from HEK 293T cells or the medium after culturing the cells. |
| priorityDate |
2010-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |