http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2682750-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1859387ae681d29644f6269645d50a78 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-557 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P3-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2012-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8aa6528a225082f748e39f929f8ac5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7ae238c9b969164b74eda64398b627c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a96e63e3ef68e95a69f620a62bdc6ac8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a756e71475ec6b4188b00d8ba6b78167 |
| publicationDate | 2018-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2682750-T3 |
| titleOfInvention | Reversible staining method of a target cell |
| abstract | An in vitro method of reversible staining of a target cell, said target cell comprising a receptor molecule on the surface thereof, with a detectable label, the method comprising: contacting a mixture of cells comprising said target cell with ( i) a receptor binding reagent, the receptor binding reagent comprising at least one binding site B, wherein the binding site B specifically binds to said receptor molecule, wherein the rate constant of dissociation (kdis) as determined by surface plasmon resonance for binding between said receptor binding reagent through the B binding site and said receptor molecule has a value of 0.5 x 10-4 s-1 or greater , the receptor binding reagent further comprising at least one binding component C, wherein the binding component C is capable of being reversibly bound to a Z binding site of a multimerization reagent, (ii) a multimerization reagent, the multimerization reagent comprising at least two Z binding sites for the reversible binding of the C binding component of the receptor binding reagent, wherein the receptor binding reagent (i) and the multimerization reagent (ii) form multivalent binding complexes that bind to said target cell, each multivalent binding complex comprising at least two of said receptor binding reagents bound to one of said multimerization reagent, providing said multivalent binding complex high avidity, with respect to said receptor binding reagent; and (iii) said detectable label bound or capable of binding to said multivalent binding complex, wherein said target cell is stained by joining said multivalent binding complex to said target cell, and wherein staining of said target cell is reversible upon rupture of the binding between said C binding component of said receptor binding reagent and said Z binding sites of said multimerization reagent, wherein said receptor binding reagent that specifically binds to said receptor molecule is selects from the group consisting of an antibody, a divalent antibody fragment, a monovalent antibody fragment, a proteinaceous binding molecule with antibody-like binding properties and an MHC molecule, wherein said target cell is a mammalian cell . |
| priorityDate | 2011-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 447.