| abstract |
A polypeptide library comprising a plurality of different polypeptides, comprising: i) an antibody heavy chain (VH) variable region comprising a framework region that is at least 95% identical to the IGHV3- framework region 23 as set forth in SEQ ID NO: 3; and i) an antibody light chain (VL) variable region comprising a framework region that is at least 95% identical to the framework region of any one of IGLV1-40 (as set forth in SEQ ID NO: 18), IGLV1-44 (as set out in SEQ ID NO: 21), IGLV1-47 (as set out in SEQ ID NO: 24), IGLV3-1 (as set out in SEQ ID NO : 6), IGLV3-19 (as set forth in SEQ ID NO: 27), IGLV6-57 (as set out in SEQ ID NO: 12); wherein VH and VL are capable of forming an antigen binding site; and wherein at least two of the polypeptides differ from each other in the amino acid sequence present in one or more complementary determining regions (CDR) in the VH and / or VL variable regions, and in which the polypeptides they are soluble under reducing conditions, which are not sufficient for the oxidation of sulfhydryl groups (-SH) in a protein and are not permissible for the formation of disulfide bonds. |