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filingDate 2013-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber ES-2676439-T3
titleOfInvention Isolation procedure of microorganisms in a culture medium and associated device
abstract Procedure for the isolation of at least one microorganism from a sample susceptible to being contaminated by said microorganism, comprising the following steps: (a) providing a device for the isolation of microorganisms comprising * a waterproof lower layer * a nutrient layer, arranged on the lower layer, which comprises a support containing a dehydrated culture medium, said nutritive layer being obtained by impregnation, preferably dry, of said support by the dehydrated culture medium, and then calendered * an insulation layer permeable to the elements included in the nutritive layer, capable of retaining bacteria on its surface and covering all or part of the nutritive layer * a protective upper layer (b) depositing a certain volume of the sample on the isolation layer (c) isolating the microorganisms by exhaustion or by covering the sample with the help of an isolation medium (d) i incubating the device for a predetermined time and at a temperature that allows the growth of microorganisms, said procedure also comprising at least one step of rehydration of the culture medium with a predetermined volume of liquid before or simultaneously with step b) and / or c) and / od), preferably before or simultaneously with step b) and / oc).
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