http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2667573-T3
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_06efec7041ae4c2c86f3e5162771c114 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-33 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0665 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0037 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 |
filingDate | 2013-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19dd488b4803b9612d0a7a6cce8e99e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5371801c5154c97f1c9f8c29701df7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9d62c63c32a3d20e31b5f369a8b227d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcd511b3639dc795865dd9658684fb6c |
publicationDate | 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | ES-2667573-T3 |
titleOfInvention | Nutrient-enriched medium for HUTC growth |
abstract | A method for culturing cells derived from umbilical cord tissue comprising: a. culturing cells derived from umbilical cord tissue seeded in microcarriers in a culture medium comprising amino acids, vitamins, salts, nucleosides, lipoic / thioctic acid, ethanolamine, insulin, transferrin, sodium selenium, in which the culture medium is supplemented with serum, for a period of time sufficient to allow the cells to reach a desired initial population density; b. add a serum-free nutrient solution, after the cells have reached the desired initial population density, in which the serum-free nutrient solution comprises amino acids, vitamins, salts, insulin, transferrin, ethanolamine, lipoic acid / thioctic acid, selenium sodium, after the cells have reached the desired initial population density; and c. culturing the cells for a period of time sufficient to allow the cells to reach a desired final population density; in which cells derived from umbilical cord tissue are isolated from human cord blood tissue substantially free of blood, are capable of self-renewal and expansion in culture, have the potential to differentiate, express CD13, CD90, HLA-ABC, and not they express CD34, CD117 and HLA-DR, and in which the nucleosides are at least 0.0001 g / l of thymidine, and at least 0.005 g / l of each of adenosine, cytidine, uridine and guanosine. |
priorityDate | 2012-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1141.