http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2664873-T3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e784c2b63c6d86dabbd1ab9e85e33e1e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate | 2012-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05f4539243de45ea33eee8ea4a2f704b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2190d6178738ae4f84014da1d2b9f7ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8ab47b046c2d434a72df0501c57213e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_763104a67a0fa326fd478f08671b0f30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a7220c2973f8e6754ac144c0a4cfa0d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58c08b03485ffba2d2ad30fc5816536f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67a311f2536fd98e60ea47fbc75512ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e45f518755d6127f3d12950e51cf2c4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b71105ecf08fc294a38a6a1908797e1 |
| publicationDate | 2018-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2664873-T3 |
| titleOfInvention | Preparation process for guanylate cyclase C agonists |
| abstract | A method of preparing a peptide comprising a GCC agonist sequence selected from the group consisting of SEQ ID NOs: 1-44 and 99-107, in which the GCC agonist sequence is n amino acid units in length, with the N-terminal unit at position 1 and C-terminal unit at position n, the method comprising: providing a first fragment having a first sequence of amino acid units from position j to position k of the agonist sequence of GCC, in which j is an integer between 1 and n-1, k is an integer between 2 and n and is greater than aj, and the first fragment is protected except for an amino group of the amino acid unit at position j, or alternatively, a carboxyl group of the amino acid unit at position k, providing a second fragment having a second sequence of amino acid units from position h to position j-1 of the GCC agonist sequence or which has a third sequence of amino acid units from position k + 1 to position m of the GCC agonist sequence, in which h is an integer between 1 and n-2 and is smaller than j, m is a integer between k + 2 and n, and the second fragment is protected except by a carboxyl group of the amino acid unit at the j-1 position or an amino group of the amino acid unit at the k + 1 position, at which less one of the first and second fragments is provided through a solid phase peptide synthesis; and coupling the first and second fragments by a solution phase synthesis for the production of a protected peptide having a sequence of amino acid units from position h to position k of the GCC agonist sequence or a protected peptide having a sequence of amino acid units from position j to position m of the GCC agonist sequence, and in which the peptide is purified by a method comprising adsorption of the peptide on a polymeric adsorbent column, optionally rinsing the peptide with deionized water, elution of the peptide optionally by rinsing the polymeric adsorbent column with an aqueous solution of isopropanol to form a peptide solution, removing water and isopropanol from the peptide solution to precipitate the peptide, and optionally adding an ether to the dehydrated peptide to facilitate peptide precipitation. |
| priorityDate | 2011-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 142.