| abstract |
A monoclonal antibody that specifically binds to a human frizzled receptor selected from the group consisting of FZD8, FZD5, FZD1, FZD2 and FZD7, and that inhibits the binding of Wnt with the human frizzled receptor, wherein the antibody: (a) binds joins the sequence (K / Q) (F / Y) GF (Q / A) (SEQ ID NO: 69) and / or the sequence Q (DE / ED) AGLEVHQF (Y / W) PL (SEQ ID NO: 24) inside the human frizzled receiver; (b) in a competitive binding assay, compete for specific binding to human FZD8, FZD5, FZD1, FZD2 or FZD7 receptors with an antibody comprising a heavy chain variable region comprising SEQ ID NO: 10 and a region light chain variable comprising SEQ ID NO: 14 or SEQ ID NO: 12 and / or (c) comprises a heavy chain CDR1 comprising GFTFSHYTLS (SEQ ID NO: 1); a heavy chain CDR2 comprising VISGDGSYTYYADSVKG (SEQ ID NO: 2); and a heavy chain CDR3 comprising NFIKYVFAN (SEQ ID NO: 3); and a light chain CDR1 comprising SGDNIGSFYVH (SEQ ID NO: 7); a light chain CDR2 comprising DKSNRPSG (SEQ ID NO: 8); and a light chain CDR3 comprising QSYANTLSL (SEQ ID NO: 9). |