http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2661981-T3
Outgoing Links
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| filingDate | 2011-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | ES-2661981-T3 |
| titleOfInvention | Protein production procedure |
| abstract | Method for producing an antibody, which comprises introducing into a CHO cell in suspension: (a) an expression vector comprising a gene fragment comprising a DNA encoding an H chain of an antibody and also comprising a pair of transposon sequences at both ends of the gene fragment, an expression vector comprising a gene fragment comprising a DNA encoding an L chain of an antibody and also comprises a pair of transposon sequences at both ends of the gene fragment and an expression vector comprising a gene fragment comprising a selectable marker gene and also comprises a pair of transposon sequences at both ends of the gene fragment, or (b) an expression vector comprising a gene fragment comprising a DNA encoding an H chain of an antibody and a selectable marker gene and also comprises a pair of transposon sequences at both ends of the fragment The gene and an expression vector comprising a gene fragment comprising a DNA encoding an L chain of an antibody and also comprises a pair of transposon sequences at both ends of the gene fragment, or (c) an expression vector comprising a gene fragment comprising a DNA encoding an L chain of an antibody and a selectable marker gene and also comprises a pair of transposon sequences at both ends of the gene fragment and an expression vector comprising a gene fragment comprising a DNA that encodes an H chain of an antibody and also comprises a pair of transposon sequences at both ends of the gene fragment, wherein each pair of transposon sequences are Tol1 nucleotide sequences or Tol2 nucleotide sequences; allow a transposase to act on the transposon sequences thereby integrating the gene fragments into a CHO cell chromosome to obtain a CHO cell expressing the antibody; and culture the CHO cell in suspension. |
| priorityDate | 2010-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1043.