| abstract |
A method for detecting a high-definition circulating tumor cell (HD-CTC) in a sample from a subject, comprising: a) providing a sample suspected of having at least one CTC and at least one non-CTC that is present at a concentration that is at least 10 times that of the CTC, b) contacting the sample with more than one detectable agent, in which the detectable agents bind to a cell marker and are two positive markers and a negative marker, in that the positive markers are cytokeratin and androgen receptor and the negative marker is CD45, and a nuclear marker and / or content marker; c) perform cellular imaging on the sample of (b) to generate a cellular image; and d) detect the HD-CTC in the sample by analyzing the image of (c), in which the HD-CTC express the positive markers, have an intact nucleus, are morphologically distinct from normal white blood cells (WBC) and are not positive for The negative marker. |