abstract |
An isolated monoclonal antibody or an antigen-binding fragment thereof that specifically binds to human IL-31, which comprises a humanized heavy chain variable domain and a humanized light chain variable domain, in the that the variable domain of the humanized heavy chain comprises a CDR1, CDR2 and CDR3, which consist of the amino acid sequences of SEQ ID NO: 1, 2 and 3, respectively, and the conserved framework regions FR1, FR2, FR3 and FR4 which have an amino acid sequence that is at least 90% identical to the amino acid sequences of SEQ ID NO: 12 (FR1), SEQ ID NO: 13 (FR2), SEQ ID NO: 14 (FR3) and SEQ ID NO: 15 (FR4), respectively; and wherein the humanized light chain variable domain comprises a CDR1, CDR2 and CDR3, which consist of the amino acid sequences of SEQ ID NO: 5, 6 and 7, respectively, and the conserved framework regions FR5, FR6, FR7 and FR8 having an amino acid sequence that is at least 90% identical to the amino acid sequences of SEQ ID NO: 17 (FR5), SEQ ID NO: 18 (FR6), SEQ ID NO: 19 (FR7) and SEQ ID NO: 20 (FR8), respectively. wherein the amino acid residue at position 29 of SEQ ID NO: 12 (FR1) is a leucine, the amino acid residue at position 8 of SEQ ID NO: 14 (FR3) is a lysine, the rest of amino acid at position 32 of SEQ ID NO: 14 (FR3) is a phenylalanine and the amino acid residue at position 15 of SEQ ID NO: 19 (FR7) is a tyrosine. |